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Data processing All data obtained from the four assays with the two systems were processed with software (Progenesis QI 3.0.3.0, Nonlinear Dynamic, Newcastle, UK) for peak picking, alignment, and normalization to produce peak intensities for retention time ( t R ) and mass-to-charge ratio ( m/z ) data pairs

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Moreover, cold priming was found to modify chloroplast to nucleus signalling by thylakoid APX-mediated suppression of CEF mediated by the thylakoid NADH dehydrogenase complex (Seiml-Buchinger et al

RESULTS Cancer cells maintain viability and proliferation in the absence of ACLY and exogenous acetate Since cancer cells are adept at engaging available metabolic flexibility mechanisms, we hypothesized that non-canonical acetyl-CoA production mechanisms could be revealed by developing ACLY KO cancer cell models that are not dependent on acetate for viability